Flow Cytometric Measurement of Cell Organelle Autophagy
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چکیده
منابع مشابه
Novel Quantitative Autophagy Analysis by Organelle Flow Cytometry after Cell Sonication
Autophagy is a dynamic process of bulk degradation of cellular proteins and organelles in lysosomes. Current methods of autophagy measurement include microscopy-based counting of autophagic vacuoles (AVs) in cells. We have developed a novel method to quantitatively analyze individual AVs using flow cytometry. This method, OFACS (organelle flow after cell sonication), takes advantage of efficien...
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The primary cilium which is an organelle in nearly every cell in the vertebrate body extends out of the cell surface like an antenna and is known as cell sensor of mechanical and chemical stimuli. In previous numerical simulations, researchers modeled this organelle as a cantilevered beam attached to the cell surface. In the present study, however, we present a novel model that accommodates for...
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Background: The B-cell defect in X-linked agammaglobulinemia (XLA) is caused by mutations in the gene for Bruton's tyrosine kinase (BTK). BTK mutations result in deficient expression of BTK protein in peripheral blood monocytes. Methods: Using the anti-BTK monoclonal antibody (48-2H), a flow cytometric analysis of intra cytoplasmic BTK protein expression in monocytes was performed to identify I...
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تاریخ انتشار 2017